Myanmar Health Sciences Research Journal
Volume 30, Number 2
TITLE: Phylogenetic Analysis of Human Respiratory Syncytial Virus from Children with Acute Respiratory Infection Admitted to Yangon Children’s Hosp
AUTHOR: Kay Thi Aye, Lay Myint Yoshida, Aung Zaw Latt, Hlaing Myat Thu, Theingi Win Myat, Hsu Htet Thwe, Sandar Aung, Ye Myint Kyaw & Kyaw Zin Thant
SOURCE: Myanmar Health Sciences Research Journal, Vol. 30, No. 2, 2018
ABSTRACT: Human respiratory syncytial virus (RSV) is one of the most important pathogens responsible for acute respiratory tract infection (ARI) outbreaks in children worldwide. RSV is a member of the family Paramyxoviridae in which differentiated into two groups (A and B) based on antigenic and genetic variability. To date, 11 genotypes for RSV group A and 23 for RSV group B have been described based on changes in the G gene coding for the attachment glycoprotein. In this study, nasopharyngeal swab samples were collected from hospitalized pediatric ARI cases at Yangon Children Hospital from January to September, 2014. Of 160 cases, non-structural protein 1 (NS1) gene of RSV was detected in 16.3% (26/160), comprising RSV-A strains 52% (11/21) and RSV- B strains 48% (10/21). Furthermore, 21 NS1 gene-positive nasopharyngeal swab samples were processed for genotyping by sequencing of C terminal of the G gene, second variable region. G gene of the RSV was successfully sequenced in 61.9% (13/21) of samples. RSV-A strain was the larger group, accounting for 53.8% (7/13), followed by RSV-B, 38.5% (5/13) and one case 7.7% (1/13) was a mixed infection. The phylogenetic analysis revealed that all group-A strains clustered as the ON1 genotype. Additionally, 72 nucleotide duplication in the second highly variable region of attachment G gene was observed in all RSV ON1 genotype in subgroup-A isolates. Moreover, one isolate of ON1 genotype showed G284S substitution as a novel mutation. Molecular surveillance of RSV infection should be conducted in Myanmar.
SUBJECT HEADINGS: Phylogenetic analysis, Human respiratory syncytial virus, Children with acute respiratory infection