Myanmar Health Sciences Research Journal
Volume 30, Number 1
TITLE: Insulin Receptor Substrate-1 Gene (G972R) Polymorphism and Metabolic Syndrome in Type-2 Diabetes Mellitus Patients
AUTHOR: Thae Nu Htwe1*, Myat Mon Oo2, Saw Wut Hmone3, Moh Moh Htun2,Ohnmar Myint Thein1 & Myat Thandar4
SOURCE: Myanmar Health Sciences Research Journal, Vol.30, No.1, 2018
ABSTRACT: Metabolic syndrome (MS) is one of the fastest growing health problems worldwide. It is major risk factor for both diabetes and cardiovascular disease. Many candidate gene studies have identified the linkage between MS and a number of genes. The insulin receptor substrate-1 (IRS-1) gene especially at codon 972 (G972R) is also a candidate for insulin resistance in type 2 diabetes and MS. The aim of the present study was to determine the insulin receptor substrate-1 gene (G972R) polymorphism and MS in type 2 diabetes mellitus (T2DM) patients. The genomic DNA of the 100 subjects from Diabetes Out-patients Department of YGH was amplified by polymerase chain reaction (PCR) and digested by restriction fragment length polymorphism (RFLP) with BstN1 used for codon 972. Metabolic syndrome was defined as in IDF (International Diabetes Federation) criteria. Metabolic syndrome was found in 84% (16/19) of carrier patients and 82% (67/81) of non-carrier patients. It was not significantly associated with presence or absence of IRS-1 (G972R) mutation. There was a significant difference (p=0.005) in proportion of MS between IRS-1 (G972R) non-carriers with family history of diabetes mellitus and those without. It, therefore, seems reasonable to argue that family history of T2DM does not play an important role in the development of MS in the IRS-1 (G972R) non-carrier.
SUBJECT HEADINGS: Insulin receptor substrate-1, Metabolic syndrome