Myanmar Health Sciences Research Journal
Volume 30, Number 1
TITLE: Repellency Effect of Artemisia vulgaris Essential Oil against Aedes aegypti Mosquitoes in Laboratory Condition
AUTHOR: Maung Maung Mya1*, Nwe Nwe Oo2, Aye Win Oo1, Sein Thaung1 & Yan Naung Maung Maung1
SOURCE: Myanmar Health Sciences Research Journal, Vol. 30, No. 1, 2018
ABSTRACT: The mosquito species Aedes aegypti L. is a major vector of dengue fever (DF), dengue haemorrhagic fever (DHF), Chikungunya, yellow fever and Zika fever. Dengue virus transmission (serotypes 1-4) by Aedes mosquitoes is a public health problem that principally affects tropical countries. Myanmar is one of the DF and DHF endemic areas in Southeast Asia Region. In mosquito control programs, botanical origin insecticides (pyrethrum, rotenone, sabadilla etc.) have the potential to be used as ovicidal, larvicidal and adulticidal of eggs, larvae and adult mosquitoes. Artemisia vulgaris (derived from Taunggyi Township, Shan State) dried leaves powder was subjected to hydro-distillation in a Clevenger-type distilling apparatus. The resulting oil was dried over anhydrous sodium sulphate and resulting 8 gm of essential oils were obtained from 100 gm of dried leaves powder. Mosquito repellency assay was done to test the efficacy of the essential oil against Aedes aegypti on human volun-teers. Artemisia vulgaris essential oil applied on average 342 cm2 area of arm for 100% protection against Aedes aegypti mosquitoes landing to probe the skin was found with the dose 0.08 g/ml or 0.0002 g/cm2. Repellency activity of complete protection time of Artemisia vulgaris oil dose 0.0002 g/cm2 provided 97.62% protection for 30 minutes, 92.86% protection for 60 minutes and 85.71% protection for 120 minutes against Aedes aegypti. Artemisia vulgaris leaves essential oil did not cause dermal irritation when applied to human skin. No adverse effects on human volunteers were observed after application. Essential oil of Artemisia vulgaris leaves was found to have very effective protection from biting of Aedes aegypti.
SUBJECT HEADINGS: Repellency, Dilution, Protection, Artemisia vulgaris, Essential oil