Myanmar Health Sciences Research Journal
Volume 29, Number 1
TITLE: Identification of Dengue Serotypes in Children with Dengue Infection at Yangon Children’s Hospital in 2015
AUTHOR: Win Kay Khine, Theingi Win Myat, Hlaing Myat Thu, Wah Win Htike, Khine Mar Oo, Khin Mar Aye, Mo Mo Win, Ye Myint Kyaw & Kyaw Zin Thant
SOURCE: Myanmar Health Sciences Research Journal, Vol. 29, No. 1, 2017
ABSTRACT: Dengue is the most important arthropod-borne viral infection of humans. All four dengue virus serotypes (DENV1-DENV4) were circulating in Myanmar and DENV-1 was the most frequently isolated one starting in 2001. To identify dengue serotype, a total of 234 paired serum samples were collected from patients with clinically suspected dengue infection attending Medical Wards of Yangon Children’s Hospital from January to July 2015. The convalescent serum samples (S2) were tested by Immuno-chromatographic tests. The proportion of serologically confirmed cases was 76% (178 out of 234). Out of these, 178 seropositive cases, 46(26%) were diagnosed as primary and 132(74%) as secondary dengue infection. Among 46 cases of primary infection, 24(52%) were DHF grade I, 10(22%) were grade II, grade III and grade IV were 7(15%) and 5(11%), respectively. Among 132 cases of secondary infection, 72(55%) were DHF grade I, 24(18%) were grade II, 19(14%) were grade III and 17(13%) were grade IV. DHF GI and DHF GII (mild form of dengue) were the most common in both infections. The corresponding acute phase serum (S1) of the ICT positive (S2) samples with <5 fever days were selected for virus isolation by tissue culture in C6/36 mosquito cell lines and further typed with serotype specific monoclonal antibodies to four dengue viruses by using Indirect Immunofluorescence Assay. Out of 103, dengue virus was isolated from 20 samples accounting for 19% isolation rate. Among isolated dengue viruses, 14(70%) were DENV-1, 2(10%) were DENV-2, 1(5%) was DENV-3 and 3(15%) were found to be of concurrent infections (1 case of DENV-1+DENV-2 and 2 cases of DENV-1+DENV-4). In Myanmar, since 2001 except in 2007, the predominant one was serotype 1. The present study showed that it has not changed. Concurrent infections were also detected in 2015 but they did not associate with severe form of disease.
SUBJECT HEADINGS: Serotype, Primary infection, Secondary infection, Dengue