Myanmar Health Sciences Research Journal
Volume 29, Number 1
TITLE: Comparative Study of Saline Wet Mount and Modified Kato-Katz Methods for Detection of Intestinal Helminths from Under 5-Year Children of Day Care Centres, Mingaladon Township
AUTHOR: Set Paing Htoo, Thet Naing Phyo, Khine Khine Su, Khin Maung Nyunt & Tin Maung Hlaing
SOURCE: Myanmar Health Sciences Research Journal, Vol. 29, No. 1, 2017
ABSTRACT: Saline wet mount technique is commonly used for detection of helminthic infection in most laboratories of Myanmar. But this technique is less sensitive and could not determine the intensity of helminthic infection. The modified Kato-Katz technique is the best quantitative method to detect intensity. This study was to compare sensitivity, specificity and practicability of saline wet mount and modified Kato-Katz methods. The cross-sectional, comparative study was conducted from June 2013 to July 2014. A total of 100 stool samples were taken from under 5-year children who were attending the day care centres of Mingaladon. The detection rate of intestinal helminths by formol-ether concentration technique was 30% followed by modified Kato-Katz method and direct saline wet mount method (28% and 23%, respectively). Among these methods, the formol-ether concentration technique had better detection rate for helminthic eggs. The modified Kato-Katz had 93% sensitivity, 100% specificity and 98% accuracy for detection of intestinal helminths. For saline wet mount method, the sensitivity was 77%, specificity was 100% and accuracy was 93%. The results revealed that modified Kato-Katz method was shown to be more sensitive than saline wet mount.
SUBJECT HEADINGS: Kato-Katz, Saline wet mount, Intestinal helminths, Ascaris lumbricoides, Trichuris trichiura, Enterobius vermicularis