Myanmar Health Sciences Research Journal

Archives   2016

Myanmar   Health   Sciences   Research   Journal

Volume   28,  Number   3

TITLE:   Acute Toxicity Study and Antioxidant Activity of Herbal Tonic "Ayewa"
AUTHOR:   Khin Phyu Phyu, Khine Khine Lwin, Khin Tayar Myint, Mya Mar Lar, Zaw Myo Tint, Thin Thin Wah, Kyi Kyi Myint, Win Win Maw & Mar Mar Myint
SOURCE:   Myanmar Health Sciences Research Journal, Vol. 28, No. 3, 2016
ABSTRACT:   Antioxidants from natural substances such as plants, fruits, vegetables, spices and herbs that are consumed as food or ingredients have been widely investigated for several biochemical and pharmacological properties. Most of antioxidants compounds in a typical diet are derived from plant sources. The herbal tonic medicine ({0) contains honey (ysm;&nf), Myristica fragrans Houtt (Zadeikpho), Syzygium aromaticum (Layhnyin), Piper betel leaves (Kun), Tinospora cordifolia Miers. (Sindonemanwe), Santalum album L. (Nathaphyu), Glycyrrhiza glabra L. (Nwegyo), Pterocarpus santalinus L. (Nathani), Mesua ferrea L. (Gant Kaw), Carum copticum Benth & Hook. f. (five kinds of aniseed) and salt. In the present study, the objectives were to determine the phytochemical constituents, acute toxicity and free radical scavenging activity (antioxidant activity) of the test drug to compare with standard ascorbic acid. Preliminary phytochemical constituents of the drug were tested according to the Harborne methods and physico-chemical test of the test drug was done by WHO method. Free radical scavenging activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical. Acute toxicity test was performed according to OECD guideline 423. The test drug contained flavonoids, glycosides, resins, polyphenols, carbohy-drate, amino acid, saponins, tannins, steroids/triterpene and reducing sugar. Ethanolic extract (50%) of the test drug was found to have radical scavenging activity with IC50 value of 12.39 g/ml. This herbal tonic drug was non-toxic up to maximum permissible dose of 5000 mg/kg. It could be concluded that the test drug possesses antioxidant activity in the experi-mental study. Therefore, this herbal tonic ({0) is experimentally proven as a safe and a good source of traditional medicine which should be used as tonic drug.
SUBJECT HEADINGS:   Acute toxicity, Antioxidant activity, Hebal tonic Ayewa

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