Myanmar Health Sciences Research Journal
Volume 28, Number 3
TITLE: Molecular Detection of Influenza Viruses Circulating among Children Attending Yangon Children’s Hospital, 2015
AUTHOR: Htin Lin, Hlaing Myat Thu, Mo Mo Win, Khin Mar Aye, Win Mar, Hla Myo Thu, Khin Khin Oo, Khin Sandar Aye & Thidar Kyaw
SOURCE: Myanmar Health Sciences Research Journal, Vol. 28, No. 3, 2016
ABSTRACT: This study was a descriptive study conducted at Out-patient Department (OPD) of Yangon Children’s Hospital (YCH). The objective of the study was to determine the predominant subtype of influenza virus detected in children attending YCH in 2015. A total of 67 children with influenza-like illness (ILI) attending YCH were recruited in the study and throat swabs or nasal swabs were taken from them. Viral RNA was extracted from the specimens by RNA extraction kit (QIAamp Viral RNA Mini Kit). Matrix genes of influenza A virus and influenza B virus were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) using Qiagen one step RT-PCR kit. Influenza A positive samples proceeded to subtyping by multiplex PCR detecting haemagglutinin gene of influenza A (H1N1) pdm09 virus, influenza A (H1N1) virus, influenza A (H3N2) virus and influenza A (H5N1) virus. Of 67 ILI cases, influenza A virus was detected in 6 cases (8.9%) and no case of influenza B virus was detected. Female to male ratio was 5:1. Majority of influenza virus positive cases (50%) were between 5 to 9 years of age. Five out of six positive cases were detected in July and one case in August. Of influenza A virus positive cases, 4 cases (66.7%) revealed HA gene of influenza A (H3N2) virus and 2 cases (33.3%) revealed that of influenza A (H1N1) pdm09 virus. So, the predominant subtype of influenza virus among children attending YCH in 2015 was influenza A (H3N2) virus which also prevailed among children attending YCH in 2013 and 2014.
SUBJECT HEADINGS: Influenza, Subtype, Predominant, Outbreak