Myanmar Health Sciences Research Journal
Volume 28, Number 2
TITLE: Establishment of Modified WST8/1 Methoxy PMS Method in Detection of Glucose-6-Phosphate Dehydrogenase Enzyme Deficiency
AUTHOR: Nwe Nwe Oo, Moe Thida Kyaw, Lwin Zar Maw, Tin Ko Ko Oo, Aung Myat Kyaw & Nwe Ni Aung
SOURCE: Myanmar Health Sciences Research Journal
ABSTRACT: Glucose-6-phosphate dehydrogenase (G6PD) enzyme deficiency is the most common heredity disorder in Myanmar. For screening of this disorder in community, transport of fresh blood and doing of laboratory procedures in time are the important factors. In Myanmar, many researchers tried to diagnose G6PD enzyme deficiency with different methods. All of these tests are tedious, time consuming and unsuitable for mass screening. Agarose gel electrophoresis, a semi-quantitative method routinely used for screening is also time consuming, more expensive and needs special equipment. Recently, the laboratory in Department of Medical Research (DMR) has developed modified WST8/1methoxy PMS method, a semi-quantitative method for screening purpose. In this method, blood samples were collected in Whatman 3MM chromatography paper and enzyme activity was measured by ELISA method. The aim of the study was to compare the applicability of agarose gel electrophoresis method and modified WST8/1 methoxy PMS method in community for screening of G6PD enzyme deficiency. Blood samples were collected from 600 pregnant women and 316 husbands from Antenatal Care Unit of Central Women’s Hospital from January to March in 2014. The deficient rate in Modified WST8/1 Methoxy PMS was 2.7% in female subjects and 3.8% in male subjects; in agarose gel electrophoresis method- 3.71% in females and 4.1% in males. The Kappa statistics for the agreement of results between these two tests were 0.88 (p<0.0001). The above findings showed the efficacy of modified WST8/1 Methoxy PMS method is not different with agarose gel electrophoresis method in detection of G6PD enzyme deficiency.
SUBJECT HEADINGS: G6PD, WST8/1 Methoxy PMS method, ELISA, Pregnancy