Myanmar Health Sciences Research Journal
Volume 28, Number 2
TITLE: Molecular Typing of Human Papilloma Virus (HPV) in Oral Mucosal Lesions of Potentially Malignant and Malignant Cases
AUTHOR: Moh Moh Htun, Yin Min Htun, Myat Mon Oo, Than Than Swe, Hnin Nu Htwe, Ohnmar, Hlaing Myat Thu & Swe Swe Win
SOURCE: Myanmar Health Sciences Research Journal
ABSTRACT: A cross-sectional descriptive study was performed on eighty oral mucosal lesions collected from 43 potentially malignant cases including 12 cases of oral leukoplakia, 6 cases of erythroplakia, 15 cases of oral submucous fibrosis (OSMF), 6 cases of oral lichen planus and 4 cases of other uncommon types and 37 patients of different grades of squamous cell carcinoma (OSCC) attended the Oral Clinical Unit of Dental University (Yangon) within one year. DNA was extracted from all fresh samples by using Qiagen kit USA. ADH2 gene (Alcohol dehydrogenase 2 gene) was detected in 41 cases (51.25%) at 146 bp for internal control. Samples of ADH2 positive were tested for HPV malignant and benign types by PCR. The malignant type (HPV-16) was detected in only one case of OSCC and benign types (HPV6 and 11) were found in one case of OSMF and two cases of OSCC by using PCR with specific two primer sets HPV-M and HPV-BK) Takara kit, Japan. Mean age of both sexes was 44±2.4 years in potentially malignant and 54±2.4 years in OSCC cases. Age variance between these two types was significantly different (p=0.0068)but there was no sex difference p=0.314). Both HPV types were not significantly related to different histological types of oral lesions (p=0.278 and p=0.470). HPV type 16 was detected in old female OSCC but benign types were found in two young male patients with OSMF and OSCC. The benign types more commonly occur in younger age that may persist or relapse spontaneously. HPV-positive OSCC has a better prognosis than HPV-negative OSCC because it has higher response rate to radiation and chemotherapy. Early diagnosis and treatment of HPV infection in oral mucosal lesions are more important for prevention of oral cancer.
SUBJECT HEADINGS: Oral squamous cell carcinoma, Human papilloma virus benign and malignant types