Myanmar Health Sciences Research Journal
Volume 27, Number 3
TITLE: Larvicidal Effect of Kaffir Lime (Citrus hystrix DC) Leaves Extract against Aedes aegypti Larvae
AUTHOR: Maung Maung Mya, Yin Yin Aye, Aye Win Oo, Khin Thant Zin & Yan Naung Maung Maung
SOURCE: Myanmar Health Sciences Research Journal, Vol. 27, No. 3, 2015
ABSTRACT: Attempt had been made to develop vector control using natural plant product. For this purpose Kaffir lime (Citrus hystrix DC) leaves were collected from Mawlamyine Township, Mon State. One hundred grams of dried leaves powder were extracted with 95% ethanol 1:5 wt/v by cold percolation method in laboratory for 7 days. The resulting 8 gm of viscous material was obtained from 100 gm of dried leaves. Different concentrations of ethanolic leaves extract of Citrus hystrix DC as 2.4%, 2.1%, 1.8%, 1.5% and 1.2% were prepared freshly in 100 ml each of distilled water in 150 ml plastic cups. Laboratory-reared Dagon Township strain of Aedes mosquito larvae were used for testing larvicidal properties of ethanolic leaves extract of Citrus hystrix DC Larvae were exposed 24 hours for each replication in different concentrations in laboratory. Result revealed that the highest dose 2.4% produced 99.5 % mortality followed by 2.1% crude extract with 85.5% mortality of 3rd and 4th instars Aedes larvae. And, 1.8% concentration of ethanolic leaves crude extract found 62.5% mortality of Aedes larvae within 24 hours. The effective lethal concentrations of LC50 for 50% mortality and LC90 for 90% mortality were found to be 1.73% and 2.08% crude ethanolic leaf extract of Citrus hystrix. Beta-citronellal (66.85%) was the most abundance compound in the leaf of Citrus hystrix, which might be toxic to the tested mosquito larvae. In conclusion, the ethanolic leaves extract of Citrus hystrix exhibited strong larvicidal activity against Aedes larvae which could be considered as a potentially alternative source for developing novel larvicides to be used in controlling vectors of mosquito-borne disease.
SUBJECT HEADINGS: Citrus hystrix, Aedes larvae, Concentration, Mortality, Ethanolic extract