Myanmar Health Sciences Research Journal
Volume 12, Number 1-3
TITLE: The role of lepro-agglutination test for serodiagnosis of leprosy in Myanmar.
AUTHOR: Khin Nwe Oo; Nwe Nwe Yin; Hauk Kying; Kyaw Nyunt Sein; Kyaw Kyaw
SOURCE: Myanmar Health Sciences Research Journal. 2000; 12(1-3): 36-40
ABSTRACT: If infectious cases of leprosy could be diagnosed for effective chemotherapy before they would manifest the disease, it would significantly prevent the transmission of infection from man to man and consequently would greatly help the leprosy eradication programme of the country. Therefore the role of lepro-agglutination test as a serodiagnostic assay of leprosy in our community was carried out in this study. It was carried out from August 1999 to January 2000. After getting informed consent, 2 ccs of blood were collected from 101 fresh leprosy cases from Central Special Skin Center, Yangon General Hospital, 178 contacts of above cases, 100 controls from National Blood Bank by using disposable needles and syringes. Sera were separated from clotted blood by centrifugation at 3000 rpm for 15 min and they were stored at -20Ã‚Â°C before testing. Lepro-agglutination (LA) test and ELISA test were carried out on these sera samples. The sensitivity of multibacillalry (MB) and paucibacillary cases of LA test was 81.13% and 56.25% respectively and the specificity for controls was 99% in this study. Both LA and ELISA tests were more sensitive in diagnosis of MB cases. Interruption of leprae transmission in areas of predominantly MB disease may be needed more due to the relatively greater number of cases acting as sources of infection before the disease clinically manifested. Therefore, in such areas there may be a role for PGL-t serology and LA test in leprosy control.
SUBJECT HEADINGS: Agglutination Tests. Serologic Tests. Leprosy.