Myanmar Health Sciences Research Journal
Volume 12, Number 1-3
TITLE: Russell's viper (Daboia russelii siamensis) toxoid: variation in biological properties of stored toxoid.
AUTHOR: Aye Aye Myint; Tun Pe; Kyi May Htwe
SOURCE: Myanmar Health Sciences Research Journal. 2000; 12(1-3): 7-9
ABSTRACT: Biological properties of formalin-treated Russell's viper venom toxoid stored at +4Ã‚Â°C was monitored by using the WHO recommended methods at 2,3,0,10 and 12 months following preparation. Formal toxoiding of the venom resulted in reduction of 15- fold in lethal, 4- fold in coagulant, 22- fold in defibrinogenating, 6- fold in haemorrhagic and 10- fold in necrotic activities. A 44- fold reduction in capillary permeability increasing activity of the toxoid was observed from 4 months onwards. Further two-fold reduction in haemorrhagic and necrotic activities of the toxoid was noted from 6 months onwards. Decrease in number of protein bands in SDSÃ‚Â¬PAGE electrophoresis was observed when the toxoid aged. Such variation in biological properties of stored toxoid will influence the quality of antibody produced and hence the protective immunity of the immunised subjects.
SUBJECT HEADINGS: Russell's Viper. Viper Venoms.