Myanmar Health Sciences Research Journal
Volume 23, Number 2
TITLE: Evaluation of antidiarrhoeal activity of Swertia angustifolia Ham. On Mice.
AUTHOR: May Aye Than; Mu Mu Sein Myint; Myint Myint Khine; Phyu Phyu Win; San San Myint; Nu Nu Win; Naw Ohnmar Tun
SOURCE: Myanmar Health Sciences Research Journal. 2011; 23(2): 108-115
ABSTRACT: In order to combat the problems of diarrhoea globally, the World Health Organization encouraged studies for the treatment of diarrhoea diseases with herbs. It thus becomes important to evaluate commonly available natural drugs as alternative to currently used antidiarrhoeal drugs.The study was carried out to evaluate acute toxicity, phytochemical, and antidiarrhoeal activity of Swertiaangustifolia. Acute toxicity study showed that there was no toxic effect even with maximum permissible dose of 24 g/kg. No toxic compound and metal like cyanogenic glycoside and arsenic were detected. In vivo antidiarrhoeal activities of aqueous extracts (3, 6, 12 g/kg single oral doses) were carried out using castor oil-induced diarrhoea model in mice with standard loperamide. Like loperamide, all doses of extract produced significant reduction in fecal output and prolongation in onset of diarrhoea (p<0.05-0.0005) compared with that of control. To understand the mechanism of action, it is also evaluated castor oil-induced gastrointestinal motility (intestinal-transit) and intestinal fluid accumulation. The extract and loperamide also significantly reduced the castor oil-induced gastrointestinal motilityand intestinal fluid accumulation (anti-secretory effect) (p<0.05-0.0005). The in vivo antidiarrhoeal index of extract and loperamide were 60.2% and 97.4%, respectively. Antibacterial activity screening of aqueous extracts against 9 types of pathogenic microorganisms was carried outby employing agar disc diffusion method. It inhibited the growth of enterotoxigenic Escherichia coli (ETEC), Salmonella typhi and Salmonella paratyphi. These results indicated their potential usefulness in infected diarrhoea with these three organisms. In conclusion, aqueous extract of Swertia angustifolia revealed the antidiarrhoeal effect and potential utility in both infected and non-infected diarrhoea states.
SUBJECT HEADINGS: Antidiarrheal Agents. Plants, Medicinal.