Myanmar Health Sciences Research Journal
Volume 24, Number 2
TITLE: Empirical optimization of agitation rate for industrial fermentation of H.polymorpha yeast (recombinant hepatitis B vaccine product).
AUTHOR: Khin Khin Aye; Khin Pyone Kyi; Sandar Nyunt; Moh Moh Htun; Win Aung
SOURCE: Myanmar Health Services Research Journal. 2012; 24(2): 79-83
ABSTRACT: Recombinant hepatitis B vaccine was produced by using the HBsAg- expressed Hansenula polymorpha yeast cells. The sufficient data of optimized agitation rate for fermentation were not available in production of recombinant hepatitis B vaccine in Myanmar. This study looked into the three lots of fermentation conditions. The yeast fermentation was performed at various agitation rates of 200, 250, 300, 350, and 400 rpm with constant fermentation conditions: temperature of 25Ã‹Å¡C, air flow rate of 70 l/min, and vessel pressure of 1.9 bars. Methanol feeding was done to induce more antigen expression and cell growth during fermentation process. The samples were collected eight hourly and were determined for the OD600nm, the cell growth by microscopic examination and the weight of biomass. It was found that the fermentation agitation rate of rpm 250 to 400 in step-wise changing (rpm 250-300-350-400 after every second time of methanol feeding) was the best method for industrial fermentation of H. polymorpha yeast in production of recombinant hepatitis B vaccine.
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