Myanmar Health Sciences Research Journal

Archives   2012

Myanmar   Health   Sciences   Research   Journal

Volume   24,  Number   1

TITLE:   Antihyperglycemic activity and related chemical constituents of Premna integrifolia Linn. (Taung-Tan-Gyi).
AUTHOR:   Khin Tar Yar Myint; Saw Hla Myint; Thaw Zin; May Aye Than; Mu Mu Sein Myint; Myint Myint Khine; Mar Mar Myint; San San Myint
SOURCE:   Myanmar Health Services Research Journal. 2012; 24(1): 35-40
ABSTRACT:   The aims of the study were to evaluate the antihyperglycemic activity of Premna integrifolia Linn. (Taung-Tan-Gyi) and to identify the chemical constituent(s) from active extracts. Using standard glibenclamide (4 mg/kg) as positive control, 70% EtOH extracts of various parts (leaves, stem barks and roots) (4 g/kg) were tested for antihyperglycemic activity on adrenaline-induced rat model. Blood glucose levels of rats at various time intervals were measured by glucometer. All extracts showed blood glucose lowering effect, with leaves extract possessing antihyperglycemic activity at 1 hr (p<0.01) while stem bark and root extracts at 2 hr (p<0.005) and 3 hr (p<0.05), respectively. The most active stem bark extract was further fractionated to chloroform-soluble and insoluble portions, and antihyperglycemic activity tested. Chloroform-insoluble fraction showed significant blood glucose lowering effect at the dose of 2 gm/kg (that is less than crude 70% EtOH extracts) at 2 hr (p<0.05) and 4 gm/kg dose (that is the same dose of crude 70% EtOH extracts) at 1 hr (p<0.05), 2 hr (p<0.005) and 3 hr (p<0.05), respectively. Column chromatographic separation of this active extract (chloroform-insoluble fraction of 70% EtOH extracts of stem bark) yielded and led to the isolation of a pure compound. The compound was chemically identified to be “aphelandrine” (C28H36N4O4) by means of UV, FT-IR, 13CNMR, 1HNMR, HMQC, HMBC, COSY and mass spectroscopy.

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