Myanmar Health Sciences Research Journal


Archives   2012

Myanmar   Health   Sciences   Research   Journal

Volume   24,  Number   1

TITLE:   Bacterial profile, drug sensitivity and virulence gene patterns of diarrheagenic Escherichia coli from diarrhea cases in Magway Township.
AUTHOR:   Nyein Nyein; Kyi Kyi Thinn; Khin Nwe Oo; Wah Wah Aung
SOURCE:   Myanmar Health Sciences Research Journal. 2012; 24(1): 7-13
ABSTRACT:   A total of 359 stool samples and 559 rectal swabs from patients with diarrhea admitted to Magway Regional Hospital from 2007 to 2009 were screened for diarrheagenic Escherichia coli (Esch. coli) using conventional laboratory methods, and polymerase chain reaction (PCR). Out of the 93 Esch. coli isolates, serotyping identified 28 enteropathogenic Esch. coli (EPEC), 13 enterotoxigenic Esch. coli (ETEC) and 8 enteroinvasive Esch. coli (EIEC). Forty-four isolates were nontypable. PCR assay showed only 5 EPEC, 8 ETEC and 8 enterohaemorrhagic Esch. Coli (EHEC) patho-type. The primers used in PCR assay were LTI-01 and LTI-02 for ETEC-labile toxin, ST-157 and ST-158 for ETEC-stable toxin, STX-01, STX-02, STX-101 and STX-102 for EHEC and eae 01 and eae 02 for EPEC. Enterotoxin production tests were performed only in 44 Esch. coli isolates. Latex agglutination test was done for verotoxin (VT) and LT, and ELISA for ST. Toxin assays showed 10 isolates produced VT, 22 isolates produced ST and only one isolate produced both VT and ST. The antibiotic susceptibility test showed that 71% of the isolates were sensitive to cefoperazone and 47%, 42%, 36%, 32% and 32% were sensitive to gentamicin, norfloxacin, flucloxacillin plus amoxicillin, chloramphenicol and amikacin, respectively. However, 90% and 82% of the diarrheagenic Esch. coli isolates were resistant to ampicillin and co-trimoxazole, respec-tively. This study pointed out that most of the isolates were susceptible to cefoperazone. In this study, the agreement between serotypes and the virulence genes detected by PCR was not significant. Therefore, serotyping alone is not considered a sufficient method for categorizing the diarrheagenic Esch. coli strains.
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