Myanmar Health Sciences Research Journal

Archives   2013

Myanmar   Health   Sciences   Research   Journal

Volume   25,  Number   2

TITLE:   Detection of Burkholderia pseudomallei in Patients with Suppurative Infections Attending the Yangon General Hospital and New Yangon General Hospital
AUTHOR:   Than Htun; Mo Mo Win; Thaung Hla; Theingi Win Myat; Naing Lin; Thin Thin Wah
SOURCE:   Myanmar Health Sciences Research Journal. 2013; 25(2): 114-119
ABSTRACT:   Pus and blood samples were collected from 85 patients those attending the Yangon General Hospital 55 patients (32 males and 23 females) and the New Yangon General Hospital (30 patients: 12 males and 18 females) from October 2008 to September 2009 with different superlative infections including 34 cases of secondary wound infections, 15 cases of abscesses, 14 cases of ulcers, 6 cases each of burns and gangrene, 5 cases of cellulites, 4 cases of carbuncle and 1 case of bed sore. The age of the patients ranged from 12 years to 81 years. Ashdown’s agar, MacConkey agar and Nutrient agar were used for isolation of Burkholdieria pseudomallei and other bacterial pathogens. Specific biochemical tests were done for identification. Burkholdieria pseudomallei antiserum was used for serotyping. The anti-biotic sensitivity test was done by Kirby-Bauer disc agar diffusion method. Sixty-three (74.11%) out of 85 samples, yielded bacterial pathogens by culture method. Among them, Pseudomonas species were isolated from 16 cases (25.39%). Specific biochemical tests and serotyping with Burkholdieria pseudomallei antiserum were done on all 16 cases of pseudo-monas species isolated. The biochemical reaction and serotyping revealed one isolate of Burkholdieria pseudomallei. In two cases, although the biochemical tests were suggestive of Burkholdieria pseudomallei, serotyping showed only a weak positive reaction. The antibiotic sensitivity pattern of Pseudomonas species isolated was as follows; ciprofloxacin (50%), cefotaxime (43.75%), chloramphenicol (43%), gentamycin (41.25%), erythromycin (31.20%) and amoxicillin/ clavulinic acid (25%). The other pathogens isolated were Staphylococcus aureus (21 isolates), Escherichia coli (14 isolates), Klebsiella pneumoniae (7 isolates) and proteus species (4 isolates). This study was carried out to detect Burkholdieria pseudomallei among the patients with suppurative infectious.

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