Myanmar Health Sciences Research Journal

Archives   2013

Myanmar   Health   Sciences   Research   Journal

Volume   25,  Number   2

TITLE:   Application of Ki-67 Immunoexpression in Various Histopathological Stages of the Oral Mucosal Lesions.
AUTHOR:   Yi Yi Myint; Sann Sanda Khin; Wai Wai Khaing; Thar Htet San; Phyu Synn Oo; Myo Htet Htet Khaing; Khin Win Swe; Htun Naing Oo
SOURCE:   Myanmar Health Sciences Research Journal. 2013; 25(2): 97-101
ABSTRACT:   We investigated the application or Ki-67 immunoexpression for cell proliferating status in various histopathological stages or the oral mucosa from 40 volunteers (chronic betel chewer with toluidine blue test positive), who lived in Chauk Township, Magway Region, Standard immuneperoxidase method was used for Ki-67 immunostaining and the stained slides were examined microscopically using semiquantitative criteria. Ki-67 immunoexpression was positive in 35 (87.5%) cases. There was no inmmunoexpression of Ki-67 in normal mucosa apart from very few parabasal cells. In epithelial hyperplasia, Ki-67 mainly located in the suprabasal cells and there was increased in numbers of positive cell layers, Dysplastic epithelium showed varying degrees of Ki-67 immunoexpression with characteristic two phase appearance. Moreover, the positivity of Ki-67 immunoexpression was significantly increased in parallel with severity or oral lesion and high incidence of chronic betel chewing habit (97.5%) is associated with abnormal oral mucosal changes. In conclusion, this study could demonstrate that the immunoexpression or Ki-67 proteins ill various histopathological stages of oral mucosa from chronic betel chewers contributes to develop a useful adjunct to the routine diagnostic standard haematoxylin and eosin examination in screening for early premalignant and malignant lesions.

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