Myanmar Health Sciences Research Journal
Volume 25, Number 1
TITLE: In vitro susceptibility status of Plasmodium falciparum to antimarial druds and prevalence of its pfmdrl 86 polymorphism in Kawthaung.
AUTHOR: Kay Thwe Han; Kyin Hla Aye; Myat Phone Kyaw; Ye Htut
SOURCE: Myanmar Health Sciences Research Journal(Golden Jubilee Publication). 2013; 25(1): 50-55
ABSTRACT: Emergence of drug resistance in Plasmodium falciparum is a serious public health problem. To monitor the antimalarial sensitivity status and prevalence of pfmdr1 86 polymorphism of P. falciparum in Kawthaung, venous blood samples of 25 uncomplicated falciparum malaria patients were collected during malaria transmission season of 2011. In vitro drug susceptibility tests were conducted according to WHO guidelines (2000) and pfmdr1 86 polymorphism was detected by PCR/RFLP (polymerase chain reaction followed by restriction fragment length polymorphism) method. Geometric mean of effective concentration (EC50) was 13.41 nmol/l in artesunate (AS), 42.86 nmol/l in amodiaquine (AMQ) and 2193.7 nmol/l in chloroquine (CQ). Resistance rate was 40% in AS and cent% in both CQ and AMQ. Prevalence rate of wild type pfmdr1 86Asn was 64% (n=16) and multiclonal infection (carrying both wild and mutant types) was detected in 36% (n=9). Among AS resistant isolates, 80% carried pfmdr1 86 Asn (wild type). In vitro resistance rate of AS and prevalence of pfmdr1 86NY multiclonal infection were found increased in comparison to the findings of 2005-2006.
SUBJECT HEADINGS: Plasmodium falciparum. Antimarials. pfmdrl 86 polymorphism