Myanmar Health Sciences Research Journal
Volume 25, Number 1
TITLE: Development of an Enzyme Linked Immunosorbent Assay (ELISA) for determination of IgG antibody to Helicobacter pylori.
AUTHOR: Yi Yi Kyaw; Thuzar Myint; Khin May Oo; Khi Nwe Oo; Thaung Hla; Mya Mya Aye
SOURCE: Myanmar Health Sciences Research Journal (Golden Jubilee Publication).2013; 25(1): 18-23
ABSTRACT: Helicobacter pylori (H. pylori) is one of the main causes of gastritis and is highly related to gastric and duodenal cancer. Different methods are introduced for diagnosis of bacterial infections but serological tests are more common. This study developed a sensitive and specific noninvasive partial in-house enzyme immunoassay for detection of Helicobacter pylori antibody in sera of H. pylori infected individuals. H. pylori whole-cell lysate, supplied by the Bacteriology Department, Okayama University, Japan was used to demonstrate the presence of target antibody in serum samples. Peroxidase-labeled antihuman IgG was used as the second antibody. Endoscopic biopsy specimens of 59 individuals (30 males and 29 females) with dyspeptic symptoms were evaluated for H. pylori infection with histologic findings of biopsy specimens as the gold standard for diagnosis. With the assumption that the demonstration of H. pylori in biopsy specimens stained with GiemsaÃ¢â‚¬â„¢s stain as 100% accurate, the sensitivity and specificity of partial in-house enzyme immunoassay were 83% and 27%, respectively. When further evaluation was done with URINELISA on the urine samples of same patients, the sensitivity and specificity of the partial in-house enzyme immunoassay were 88% and 55%, respectively. The partial in-house enzyme-linked immunoassay greatly reduced the cost for mass screening and it is easy, rapid, non-invasive and accurate. Further evaluation of the partial in-house EIA should be carried out using a commercial EIA for H. pylori antibody as the gold standard.
SUBJECT HEADINGS: Enzyme Linked Immunosorbent Assay. IgG antibody. Helicobacter pylori