Myanmar Health Sciences Research Journal
Volume 27, Number 2
TITLE: Cardioprotective Effect of Seed Juice Extract of Punica granatum Linn. (Tha-Le) on Isoproterenol-induced Myocardial Infarction in Albino Rats
AUTHOR: Nwe Nwe Than, Nu Nu Aye, Kyae Mone Htwe, Lei Lei Win, Khin Mi Mi Lay, Khaing Khaing Mar & Khin Phyu Phyu
SOURCE: Myanmar Health Sciences Research Journal. 2015; 27(2): 130-136
ABSTRACT: This study was designed to determine the phytochemical constituents and evaluate the cardio protective effect of seed juice extract of Punica granatum Linn. On isoproterenol-induced myocardial infarction in rats. Albino rats (250-290 gm), both sexes were divided into six groups (n=6); normal control (D/W, group I), isoproterenol (ISO)-induced pathogenic control (group II), test groups which were given seed juice extracts (100 mg/kg, group III), (200 mg/kg, group IV) and (300 mg/kg, group V), respectively, and standard vitamin E (100 mg/kg, group VI) orally for 21 days. From group II to group VI, myocardial infarction was induced by ISO (85 mg/kg) subcutaneously on 20th and 21st day, consecutively. On 22nd day, all rats were anaesthetized and blood samples were collected by cardiac puncture for biochemical assay, i.e., serum AST, CK-MB, oxidative stress marker, malondialdehyde (MDA) and activity of endogenous antioxidant enzyme, glutathione peroxidase (GPx). Myocardial infarct (MI) size was measured by using NIH Image J software. Phytochemical analysis showed the presence of flavonoids, phenolic compounds which have antioxidant properties. Statistical analysis was performed with ANOVA. There were significantly increased (p<0.05) serum AST, CK-MB, MDA and depletion of antioxidant GPx in pathogenic control group when compared with normal control group. Pretreatment with seed juice extracts (200 and 300 mg/kg) and vitamin E (100 mg/kg) groups significantly decreased (p<0.05) the AST, CK-MB, MDA, MI sizes and significantly increased (p<0.05) the GPx when compared with pathogenic control group. The test group V (extract 300 mg/kg) and standard vitamin E group are comparable in their effectiveness. In conclusion, Punica granatum Linn. Possess cardio-protective effects probably due to presence of phenolic compounds, augmen-tation of the endogenous antioxidants and reduction of oxidative stress.
SUBJECT HEADINGS: Cardioprotective, Punica granatum Linn. Isoproterenol, Myocardial infarction, Albino rats