Myanmar Health Sciences Research Journal
Volume 27, Number 1
TITLE: Isolation and identification of Japanese Encephalitis Virus from Piglets in Thakayta Township, Yangon
AUTHOR: Aung Zaw Latt; Khin Mar Aye; Hay Mar Win; Khaing Moe Aung; Khin Khin Oo; Hla Myo Thu; Mo Mo Win; Hlaing Myat Thu; Sutee Yoksan
SOURCE: Myanmar Health Sciences Research Journal. 2015; 27(1): 1-6
ABSTRACT: Japanese encephalitis is a vector-borne zoonotic disease caused by the bite of Culex mosquitoes. Japanese encephalitis virus circulates among the wild birds, and transmitted to porcine, equine and human. In 2010, there were two reported cases of Japanese encephalitis infection in Thakayta Township, Yangon and the study was initiated within a month to get the JE virus isolate. A small pig farm was selected which was close (<2 km) to the home of JE cases. Blood samples were collected from five piglets in a selected pig farm, weekly for 14 times. JE virus was isolated in C6/36 mosquito cells, identified by Immunofluorescent Assay and Reverse Transcriptase- Polymerase Chain Reaction, and then sequenced by DNA sequencer. The isolate was found to be genotype I. In 2009, the first JE virus isolate in Myanmar was genotype III. This study was the second isolation of JE virus from piglets in Myanmar.
SUBJECT HEADINGS: Japanese encephalitis, Virus isolation, Gene sequencing, Phylogenetic tree