Myanmar Health Sciences Research Journal
Volume 26, Number 3
TITLE: Antimicrobial Susceptibility Pattern of Extended-Spectrum Beta Lactamase Producing Enterobacteriaceae in Urinary Tract Infection
AUTHOR: Khin Thawtar Shein;Phyu Phyu; Thida
SOURCE: Myanmar Health Sciences Research Journal. 2014; 26(3): 214-219
ABSTRACT: Extended-spectrum beta-lactamases (ESBLs) arc enzymes conferring broad resistance to penicillin, cephalosporin and monobactam and which are inhibited by clavulanic acid. These enzymes arc produced by Enterobacteriaceae. The study was performed to determine the antimicrobial susceptibility pattern of LSBL-producing Enterobacteriaceae in urinary tract infection. It was a hospital-based, cross-sectional descriptive study on patients from Mandalay General Hospital during the period of January to September. 2012 with clinically diagnosed urinary tract infection with or without urinary catheter. Phenotypic confirmatory test for ESBL production was done by using disks containing cefotaximc and ceflazidime alone and in combination with clavulanic acid. Antimicrobial susceptibility test was done by the disk diffusion method. From 113 urine specimens, 67(593%) were culture positive and 4(1(40.7%) were culture negative. Of the 67 positive urine cultures. Enterobacteriaceae were identified from 60 specimens (89.6%). Among of isolates of Enterobacteriaceae. 31 (51.7%) were confirmed as FSBLs producers. Out of 31 ESBL-producing Enterobacteriaceae. 9o.7'ro of isolates were sensitive to imipcnem, 93.5% were sensitive to amikacin. 01.3% were sensitive to cefoperazone/ sulbactum, 22.6% were sensitive to gentamicin. 10.1 % were sensitive to trimethoprim-sulfamethoxuzole and totally resistant to amoxicillin/ clavulanic acid ciprofloxacin. cefepime, ceftazidime, cefotaxi me and aztreonam. This study showed that ESBIs production was found in about half of the (I () isolated Enterobacteriaceae. As most of ESBLproducing isolates arc resistant to many classes of antibiotics, it is important to control such strains closely in order to prevent and reduce their spread.
SUBJECT HEADINGS: ESBL-producing Enterobacteriaceae; Urinary Tract Infections; Phenotypic confirmatory Test