Myanmar Health Sciences Research Journal
Volume 26, Number 2
TITLE: Establishment of the Simple Extraction of Collagen from Some Fishes in Myanmar and Evaluation of Effectiveness in Wound Healing
AUTHOR: Lin Lin Tun; Kyaw Naing; Moh Moh Htun; Khin Khin Aye; Aye Win Oo; Myat Mon Oo; Win Aung
SOURCE: Myanmar Health Sciences Research Journal. 2014; 26(2):136-140
ABSTRACT: Collagen is major component of connective tissue found in the body. Collagen has a wide range of application in cosmetic, biomedical, pharmaceutical industrial fields. Fishery production in Myanmar is growing with a huge amount of by-product released. It can be used for collagen extraction. The aim of this research was to extract collagen from fish skins by using simple extraction method and investigate the some properties of the extracted fish collagen for the application. Collagen was extracted from the skin of Ngamyitchin (Labeo rohita) and Ngaphe (Notopterus notopterus) by using common salt (NaCl). The yields of collagen for Ngamyitchin and Ngaphe were 7.65 and 6.45%, respectively. The effect of pH and salting out on the sample were determined. The maximum solubility of collagen of Ngamyitchin and Ngaphe was observed at pH 2 and 3, respectively. No changes in solubility were observed in the presence of NaCl up to 3% (w/v). However, a sharp decrease in solubility was found above 3% NaCl. Fourier transform infrared (FTIR) spectra of both samples showed the presence of N-H, O-H, C=O, -CH2, -CH3 groups in the samples. FTIR spectra of the collagen sample from both fishes showed to coincide with typical protein spectra. In this study, in vivo study was carried out for the determination of wound healing in 24 Wistar rats. Wound was induced under anesthesia in every rat and treated with collagen 1 mg/kg dressing for 18 days. Histological results showed that extracted collagen from Ngamyitchin was more completed wound healing in skin lesion than that of Ngaphe.
SUBJECT HEADINGS: Collagen; Fishes; Wound Healing