Myanmar Health Sciences Research Journal


Archives   2014

Myanmar   Health   Sciences   Research   Journal

Volume   26,  Number   2

TITLE:   Detection of Early Cases of HIV Infection by Using Fourth Generation Enzyme-Linked Immunosorbent Assay (ELISA)
AUTHOR:   Htin Lin; Hlaing Myat Thu; Khin Mar Aye; Thida Kyaw; Mo Mo Win; Khin Thet Wai; Amy Khine; Myat Min Zaw; Min Zaw
SOURCE:   Myanmar Health Sciences Research Journal. 2014; 26(2): 83-87
ABSTRACT:   Fourth generation HIV enzyme-linked immunosorbent assay (ELISA) detects both HIV p24 antigen and antibody to HIV-1 or 2 simultaneously. The aim of this study was to determine the role of fourth generation ELISA in the detection of early cases of HIV infection. It was a cross-sectional, descriptive study conducted at QC centre of PSI/Myanmar and Virology Research Division. The investigators collected 100 HIV-seronegative samples of anonymous individuals from QC centre. These individuals had possible exposure to HIV within 2 to 8 weeks before coming to the centre. When these samples were tested with fourth generation ELISA (RecombiLISA HIV antigen-antibody Test, CTK Biotech), 6 samples (6%) showed HIV positive. Two out of six samples revealed HIV-RNA, when confirmed by real time polymerase chain reaction using HIV RT-PCR kit (careHIV-1 RT-PCR Kit V2, Qiagen, Shenzhen). These two cases had the 4 weeks onset and 8 weeks onset of possible exposure to HIV previously. In conclusion, fourth generation ELISA can provide detection of some early cases of HIV infection during the window period. The results suggest the use of antigen/antibody combination assays rather than the antibody assay in the screening of HIV cases when nucleic acid amplification tests are not available.
SUBJECT HEADINGS:   HIV Infections; Enzyme-Linked Immunosorbent Assay
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