MATERIALS AND METHODS
This study was a cross-sectional, laboratory- based descriptive study in which blood samples from HIV-infected patients were collected from outpatient department of Mingalardon Specialist Hospital. The study period was from January 2016 to December 2016. Laboratory examination was done at Molecular Technology Applications Division at Department of Medical Research. A total of 150 HIV-positive patients were included in this study. Blood samples in aliquot tubes were centrifuged for 10 minutes at 2000 rpm for serum separation. After centrifugation, the serum was transferred into another sterile aliquot tube with transfer disposable pipettes and then kept in a -20°C deep freezer before testing. According to the manufacturer’s instructions, anti-HCV ELISA was done by using HCV ELISA 3.0 test kit, Standard Diagnostics Inc., Republic of Korea. Data collected from the proforma was entered and analyzed by using computerized statistical software SPSS (Statistical Package for Social Science Version 16).
This study was carried out after taking approval from Ethical Research Committee of University of Medicine 2.